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These peptides offer the distinct advantage that can be used PCNA is a potential therapeutic target in cancer therapy. In this work, we present a tool to identify cell cycle phases and in particular, sub-stages of the DNA replication phase (S-phase) based on the characteristic patterns of PCNA distribution. For Permissions, please email: journals.permissions@oup.com
ISBN 978-1-4613-7451-0. 3. pp. The unique disarticulation layer formed in the rachis of
PMID 9552415.
Cell cycle distributions of PCNA and acTUBB3 expression in P0 retinal cells as determined by flow cytometry.
Effects of herbivory and its timing on reproductive success of a tropical deciduous tree
However, besides DNA replication, PCNA functions are associated with other vital cellular processes such as chromatin remodelling, DNA repair, sister-chromatid cohesion and cell cycle control … All rights reserved. PAF15 protein levels peak in the G2/M phase of the cell cycle and drop rapidly at mitotic exit in an APC/C- and KEN-box–dependent fashion.
Published by Oxford University Press on behalf of the Annals of Botany Company. Microanatomical traits track climate gradients for a dominant C
Expression of proliferating cell nuclear antigen (PCNA)/cyclin during the cell cycle. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. • Prosperi E (1998). To eliminate the need for transfection and bypass the problem of difficult to transfect and/or short lived cells, cell permeable replication and/or repair markers can be used. MSH7 (MutSγ)Characterization of an ATP-dependent type I DNA ligase from Human 3-methyladenine-DNA glycosylase: effect of sequence context on excision, association with PCNA, and stimulation by AP endonucleaseD type cyclins associate with multiple protein kinases and the DNA replication and repair factor PCNAMolecular cloning and structural analysis of mouse gene and pseudogenes for proliferating cell nuclear antigenThe interdomain connector loop of human PCNA is involved in a direct interaction with human polymerase delta© The Author 2010. In response to DNA damage, this protein is PCNA is also involved in the DNA damage tolerance pathway known as post-replication repair (PRR).Proteins interacting with PCNA via APIM include human AlkB homologue 2, TFIIS-L, TFII-I, Rad51B,On the other hand, the study of the dynamics of replication and repair in living cells can be done introducing translational fusions of PCNA. Functional-morphological analyses of the delicate snap-traps of the aquatic carnivorous waterwheel plant (
Proliferating cell nuclear antigen (PCNA) is an evolutionarily well-conserved protein found in all eukaryotic species as well as in Archaea. doi:10.1007/978-1-4615-5371-7_15.
The expression of proliferating cell nuclear antigen (PCNA), also called cyclin, was quantified in the cell lines SP2/0 and MOLT-4 and in mouse splenocytes induced to proliferate in vitro with mitogens. Many proteins interact with PCNA via the two known PCNA-interacting motifs PCNA-interacting peptide (PIP) boxThe protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. Proliferating cell nuclear antigen (PCNA) is a protein which is involved in DNA replication and has cell cycle dependent properties. 193–210. PAF15 associates with proliferating cell nuclear antigen (PCNA), and depletion of PAF15 decreases the number of cells in S phase, suggesting a role for it in cell cycle … A–D: Plots show distribution of cells on the basis of fluorescence intensity of … Kurki P, Vanderlaan M, Dolbeare F, Gray J, Tan EM. PCNA was first shown to act as a processivity factor of DNA polymerase δ, which is required for DNA synthesis during replication (Tan et al., 1986; Bravo et al., 1987; Prelich et al., 1987). Density plots of cells triple labeled with anti‐PCNA, anti‐acTUBB3, and PI. Progress in Cell Cycle Research. Multiple roles of the proliferating cell nuclear antigen: DNA replication, repair and cell cycle control.